Demonstration of chymosin (EC 3.4.23.4) in the stomach of newborn pig.
نویسندگان
چکیده
The stomach of newborn pig contains a proteinase that is immunologically closely related to calf chymosin (rennin) (EC 3.4.23.4.). None of the pepsins from the stomach of adult pig is present in the newborn pig. Pig chymosin has optimal general proteolytic activity around pH 3.5. The ratio of milk-clotting activity to general proteolytic activity is about 30--70 times higher than that of pyloric and fundic pepsins.
منابع مشابه
Bacterial Expression and Functional Characterization of A Naturally Occurring Exon6-less Preprochymosin cDNA
Chymosin (Rennin EC 3.4.23.4), an aspartyl proteinase, is the major proteolytic enzyme in the fourthstomach of the unweaned calf, and it is formed by proteolytic activation of its zymogene, prochymosin.Following the cloning of synthesized cDNAs on mRNA pools extracted from the mucosa of the calf fourthstomach, we have identified an alternatively spliced form of preprochymosin ...
متن کاملThe primary structure of calf chymosin.
The complete amino acid sequence of calf chymosin (rennin) (EC 3.4.23.4) has been determined. The sequence consists of a single peptide chain of 323 amino acid residues. The primary structure of the precursor part of calf prochymosin was published previously (Pedersen, V.B., and Foltmann, B. (1975) Eur. J. Biochem. 55, 95-103), thus we are now able to account for the total 365 amino acid residu...
متن کاملProteins of human milk. I. Identification of major components.
Traditionally, human milk proteins are identified largely by reference to bovine milk. Hence, to identify the major proteins in human milk, we subjected human and bovine milk, in parallel, to high-resolution two-dimensional electrophoresis. Isoelectric precipitation at pH 4.6 was our criterion for distinguishing whey proteins from those of the casein complex. The alpha- and beta-caseins were id...
متن کاملPractical Economics of "accelerated" Calf Feeding Programs
Physiologically, the newborn calf is not a functioning ruminant. The abomasum, which represents the largest portion of the stomach of the newborn calf, is the primary functional unit of the gastric region. The sucking reflex allows milk to bypass the rudimentary rumen and reticulum via the reticular or esophageal groove directly into the abomasum where digestion is initiated. Milk is coagulated...
متن کاملEffect of Teaching Transportation of the Injured at the Accident Scene on Awareness, Attitude, and Performance of Soldiers by Two Methods of Role Playing and Demonstration
Introduction: The military as the first and largest incident response team in the event of unexpected accidents must be able to use their skills and speed of action to ensure the safe transfer of casualties and to prevent the aggravation of the injuries for the injured. Objective: The current study aimed to determine the effect of teaching transportation of the injured at the accident scene by ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 169 2 شماره
صفحات -
تاریخ انتشار 1978